Journal: Frontiers in Microbiology
Article Title: Comparative Transcriptomic and Metagenomic Analyses of Influenza Virus-Infected Nasal Epithelial Cells From Multiple Individuals Reveal Specific Nasal-Initiated Signatures
doi: 10.3389/fmicb.2018.02685
Figure Lengend Snippet: Individual heat-map and PCA analysis of five individual subjects show similar trends but potentially retained some degree of variability in temporal induction and magnitude between donors. (A) Individual heat-maps of different subjects revealed largely similar patterns with potential differences in temporal induction from 8 to 48 hpi, including the magnitude of induction. (B) Individual PCA plot showed that the transcriptomes across different subjects were not clustered as tightly since the hNEC samples were of different genetic backgrounds. The PCA plot showed a distinct progression of differential gene changes across time-points even at 0 hpi. (C) Individual viral titers increased over time to reach high viral loads in samples from all donors. The individual variation was deemed not clinically significant given that a sufficiently high virus titer was attained to induce a consistent response.
Article Snippet: Gene microarrays for the infected and control hNEC samples were performed using Affymetrix human transcriptome array (HTA) microarray platform.
Techniques: